England Women will be without the injured trio of Lauren James, Ella Tun and Lauren Hamp when they face Emma Hayes’ USA at Wembley on November 30.
Manchester City announced that Hamp underwent knee surgery on Tuesday, while Toon and James are both suffering from calf problems.
Chelsea forward Niamh Charles and Aston Villa defender Lucy Parker are also out.
So Sarina Wigman handed Manchester City’s Laura Blindkilde Brown and Leicester City’s 21-year-old Ruby Mays their first international senior call-ups.
Meanwhile, Manchester United defender Gabby George, who suffered an ACL injury last year, is back in the squad for the first time since November 2022.
The 24-man squad meets at St George’s Park next week before hosting the USA at Wembley later this month and Euro 2025 hosts Switzerland at Bramall Lane on December 3.
England team as a whole
Goalkeepers. Mary Earps (PSG), Hannah Hampton (Chelsea), Anna Moorhouse (Orlando Pride).
Defenders. Lucy Bronze (Chelsea), Millie Bright (Chelsea), Jess Carter (Gotham), Alex Greenwood (Manchester City), Leah Williamson (Arsenal), Esme Morgan (Washington Spirit), Millie Turner (Manchester United), Gabby George (Manchester United ): ), Maya Le Tissier (Manchester United).
Midfielders. Grace Clinton (Manchester United), Fran Kirby (Brighton), Jess Park (Manchester City), Laura Blindkilde Brown (Manchester City), Georgia Stanway (Bayern), Kyra Walsh (Barcelona) ). Ruby Mace (Leicester).
Attackers. Aggie Beaver-Jones (Chelsea), Chloe Kelly (Manchester City), Beth Meade (Arsenal), Alessia Russo (Arsenal), Jess Naz (Tottenham).